Quantification And Validation Of Vanillic Acid In Tecoma Stans Leaves By HPLC
Abstract
A simple, accurate, rapid and precise High performance liquid chromatographic (HPLC) method was developed and validated for the quantification of vanillic acid in Tecoma stans. Reverse phase Phenomenex (US) C18 Column 250 x 4.6mm I.D, 5μ Particle Size was used for the development. The mobile phase used was 0.1N potassium dihydrogen phosphate:methanol (60:40) with a flow rate of 1.0ml/min with SPD-M20A Diode Array Detector set at 247nm . The pH was maintained at 5.2. The retention time for vanillic acid was found to be 4.647. The method produced linear responses in the concentration range of vanillic acid 50%-150%. The amount of vanillic acid was found to be 5.1mg/gm. The developed method was then validated by using various validation parameters like accuracy, precision, linearity and robustness as per ICH guidelines. The method was found to be simple, specific, selective, and robust.
Keywords
References
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DOI: http://dx.doi.org/10.14259%2Fpm.v2i2.122
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