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Total phenolic composition, antibacterial and antioxidant activities of Fagara heitzii Aubr. & Pellegr. medicinal plant of Gabon

Obame Engonga, Andzi Barhé Timoléon, Ondo Joseph-Privat, Mewono Ludovic, Ndong Atome Guy Roger, Abdoul-Latif Fatouma, Bassolé Imaël Henri Nestor

Abstract


Fagara heitzii Aubr. & Pellegr is a shrub indigenous to secondary forest. The plant is widely used for its various pharmacological properties. The powdered plant samples were analyzed for their phytochemical screening using standard laboratory methods. The total phenols were measured using the Folin-Ciocalteu method. Proanthocyanidins content were determined by the method of HCl-butanol hydrolysis. Antioxidant activities were evaluated with 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The plant is rich in phenolic compounds with tannins being the most abundant compounds in the methanolic and aqueous extracts while flavonoids and proanthocyanidins are abundant in methanol and chloroform extracts. Overall, we found that the extracts tested reduced the concentration of the free radical. The oxidation of linoleic acid was weakly inhibited by methanolic and aqueous extracts of Fagara heitzii (48.8 and 39.4 % ± 0.05 of reduction). In addition, the F. heitzii extracts had a good antimicrobial activity against tested microorganisms. In the agar-well diffusion assay, the extracts are active on both Gram positive and negative bacteria. The broth microdilution assay gave minimal inhibitory concentration values ranging from 16 to 400 μg/mL. Our results suggest that Fagara heitzii has both antioxidant and antibacterial activities.


Keywords


Fagara heitzii, Antioxidant, Antimicrobial, Antiboacterial, Phenolic, Medicinal plants

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References


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DOI: http://dx.doi.org/10.14259%2Fpm.v1i3.50

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