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Spectrophotometric Method for the Estimation of Abacavir Sulphate in Bulk and Pharmaceutical Dosage Forms in Different Solvents

L Matsyagiri, Vangala Kiran Kumar, Takkadapalliwar Santoshi, Bandapalli Saritha, Pasam Pranathi

Abstract


A simple and highly sensitive UV-Spectrophotometric method has been developed for the determination of Abacavir sulphate as an anti-retro viral drug in different solvents. The proposed method is based on the measurement of light absorption in UV-region in different solvents in their different absorption spectra. Beer’s law in valid in the concentration range of 2-20 µg/ml. Abacavir sulphate exhibited maximum absorbance at pH 6.8, pH 1.2, and distilled water were 219.82 nm, 296.21 nm and 216.08 nm respectively with their molar absorptivity of 0.0091, 0.0056, 0.0095 lit/mol/cm.  The proposed method can be successfully applied for the simultaneous determinations of Abacavir sulphate in commercial tablet preparation. The method was successfully applied to marketed formulation and % purity was found to be 99.86 %. The developed method was found to be accurate, precise, repeatable, and reproducible, could be used for the routine analysis of Abacavir sulphate in bulk drug and formulations.

 


Keywords


Abacavir Sulphate, UV- Spectrophotometry, Absorbance, molar absorption co-efficient, Relative standard deviation.

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References


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DOI: http://dx.doi.org/10.14259%2Fpm.v1i3.52

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