Open Journal Systems

Cover Image

In Vitro Antimicrobial and Insecticidal Activities and their Phytochemical Estimation of Methanolic Extract and its Fractions of Perotis Hordeiformi leaves

Sajid Nabi, Abdul Manan Kakar, Alaa Bazerbashi, Yasser MSA Al-Kahraman

Abstract


The aim of the present investigation deals with biological evaluation Perotis Hordeiformi leaves. For this purpose different biological assay (agar diffusion method) of methanolic extract (Crude) and its fractions that are chloroform fraction, n-hexane fraction, Ethyl acetate fraction, n-butanol fraction and aqueous fraction were carried out. The results from the agar diffusion method indicated that Crude showed maximum antibacterial activity against Staphylococcus aureus with the inhibition zone (31.52±0.06mm). On the other hand, Crude showed maximum activity against Candida albicans and Candida glaberata with % inhibition of (74.23±0.02) and (67.53±0.11) respectively. Furthermore, Crude showed maximum insecticidal % mortality against both Tribolium castaneum and Sitophilus oryzea with (80%) and (70%) respectively. Phytochemical estimation of Crude and its fractions showed the presence of Alkaloids, Flavonoids, Phenols, tannins and Diterpenes.

Keywords


Antimicrobial; Insecticidal; Phytochemical Estimation; Perotis Hordeiformi

Full Text:

PDF

References


World Health Organization: Traditional medicine-growing needs and potential. WHO policy perspectives on medicine, No. 2. WHO, Genève 2002.

Balunas MJ, Kinghorn AD: Drug discovery from medicinal plants. Life sciences 2005, 78:431-441.

http://dx.doi.org/10.1016/j.lfs.2005.09.012

PMid:16198377

Barnes J, Anderson LA, Phillipson JD, Newall CA: Herbal medicines: Pharmaceutical Press London; 2007.

Cowan MM: Plant products as antimicrobial agents. Clinical microbiology reviews 1999, 12:564-582.

PMid:10515903 PMCid:PMC88925

Bidlack WR, Omaye ST, Meskin MS, Topham DKW: Phytochemicals as bioactive agents: CRC press; 2000.

PMCid:PMC95944

Nabi S, Ahmed N, Khan MJ, Bazai Z, Yasinzai M, Al-Kahraman YMSA: In vitro Antileishmanial, Antitumor Activities and phytochemical Studies of Methanolic Extract and its Fractions of Juniperus Excelsa Berries. World Applied Sciences Journal 2012, 19:1495-1500.

Khan MJ, Baloch NU, Nabi S, Ahmed N, Bazai Z, Yasinzai M, Al-Kahraman YMSA: Antileishmanial, Cytotoxic, Antioxidant Activities and Phytochemical Analysis of Rhazya Stricta Decne Leaves Extracts And Its Fractions. Asian Journal of Plant Science and Research 2012, 2:593-598.

Khan AA, Baloch NU, Nabi S, Khan MJ, Jamali MS, Al-Karhaman YMSA: In Vitro Antimicrobial and Insecticidal Activity Activity of Methanolic Extract and Its Fractions of Berberis Baluchistanica Roots. World Journal of Pharmaceutical Research 2012, 2:219-226.

Kakar AM, Khan AA, Nabi S, Kakar MA, Yasinzai M, Alkahraman Y: In Vitro Antileishmanial, Cytotoxic Activity and Phytochemical Analysis of Thuspeinanta Brahuica Leaves Extract and Its Fractions IJPBAS 2013, 2:520-528.

Baloch N, Nabi S, Kakar AM, Wajid Z, Al-Kahraman Y: In vitro antileishmanial, antitumor, cytotoxic activities and phyto-chemical analysis of Citrullus colocynthis fruit extract and its fractions. International Journal of Medicinal and Aromatic Plants 2013, 3:78-84.

Baloch N, Kakar AM, Nabi S, Yasinzai M, Al-Kahraman DYM: In Vitro Antimicrobial, Insecticidal, cytotoxic activities and their phytochemical analysis of Methanolic Extract and its Fractions of Peucedanum Beluchistanicum leaves. International Journal of Pharma and Bio Sciences 4:898-905.

Al-kahraman Y, Baloch N, Kakar AM, Nabi S: In Vitro Antimicrobial, Insecticidal, Antitumor, Antioxidant activities and their Phytochemical Estimation of Methanolic Extract and its Fractions of Nepeta praetervisa leaves. International Journal of Phytomedicine 2013, 4:531-536.

Baloch N, Nabi S, Al-Kahraman YMSA: In vitro Antileishmanial, Cytotoxic, Antioxidant activities and Their Phytochemical Analysis on Methanolic Extract and it is Fractions of Perotis hordeiformis leaves. International journal of Pharmacy 104:165-170.

Miles AA, Misra SS, Irwin JO: The estimation of the bactericidal power of the blood. Journal of Hygiene 1938, 38:732-749.

http://dx.doi.org/10.1017/S002217240001158X

Kavanagh F: Analytical Microbiology. Edited by F. Kavanagh: Academic Press; 1963.

Abbott WS: A method of computing the effectiveness of an insecticide. J. econ. Entomol 1925, 18:265-267.

Rahman A-u, Choudhary MI, Thomsen WJ: Bioassay techniques for drug development: Harwood Academic Publishers The Netherlands; 2001.

Collins PJ: Resistance to grain protectants and fumigants in insect pests of stored products in Australia. In Proceedings of the Australian Postharvest Technical Conference: CSIRO, Canberra, Australia: 1998:55-57.

Rashid R, Farah M, Mirza MN: Biological screening of Salvia cabulica. Pak. J. Bot 2009, 41:1453-1462.

Tabassum R, Naqvi SNH, Azmi MA, Nurulain SM, Khan MF: Residual effect of a neem fraction, nimolicine and an insect growth regulator, dimilin, against stored grain pest Callosobruchus analis. In Proceedings of the Pakistan Congress of Zoology: 1997:165-170.

Andrews JM: Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy 2001, 48:5-16.

http://dx.doi.org/10.1093/jac/48.suppl_1.5

PMid:11420333

Harborne JB: Phytochemical methods A Guide to modern techniques of plant analysis: springer; 1998.

Trease E, Evans W: Text book of pharmacognosy edn 12th. Oxford: Alden Press; 2007.

Chang C-C, Yang M-H, Wen H-M, Chern J-C: Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis 2002, 10:178-182.

Edeoga HO, Okwu DE, Mbaebie BO: Phytochemical constituents of some Nigerian medicinal plants. African journal of biotechnology 2005, 4:685-688.

Okwu DE, Josiah C: Evaluation of the chemical composition of two Nigerian medicinal plants. 2006.

Price ML, Butler LG: Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain. Journal of Agricultural and Food Chemistry 1977, 25:1268-1273.

http://dx.doi.org/10.1021/jf60214a034




DOI: http://dx.doi.org/10.14259%2Fpm.v1i3.58

Refbacks

  • There are currently no refbacks.