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Targeting lipid metabolism to improve oocyte cryopreservation (OCP) in domestic animals

Gemechu Wirtu, Justina McGill, Lindsey Crawford, Ashlyn Williams, Gopal Reddy, Werner G. Bergen, Liz Simon

Abstract


Oocyte cryopreservation (OCP) lags behind other advances that have recently been made in many areas of assisted reproductive technology. One of the risk factors that increases the chilling sensititvity, specieally in domestic animals, is the high lipid content of oocytes. Many studies have demonstrated that reducing the lipid content improves cryosurvival of oocytes. The methods that have been used to reduce the lipid content include mechanical delipidation by centrigation of the oocytes and polarizing lipid droplets, ehnahcing lipid catabolism and inhibiting lipogenesis. Targets of the latter incude fatty acids that are synthesized de novo and those taken up by the oocytes from culture media. We provide an overview of studies conducted on delipidation and suggestions for future studies in the area. 


Keywords


Oocyte, cryopreservation, lipid metabolism, delipidation, in vitro maturation

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References


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DOI: http://dx.doi.org/10.14259%2Ftcb.v1i1.60

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